Biohazardous waste must be contained in appropriate secondary containers prior to disposal.

_{The California Medical Waste Management Act}

Biohazardous and medical waste must be placed in ‘red bags’ which are located within approved secondary containment. These waste receptacles are in addition to the non-hazardous waste bins used within the laboratory. Sufficient floor space must be planned in order to have enough room for the necessary waste containers.

All BSL2 laboratories shall have vacuum lines which are protected with liquid disinfectant traps and high efficiency particulate air (HEPA) filters or filters of equivalent or superior efficiency. (Note: Filters must be maintained and routinely replaced, as necessary).

_{CCR, Title 8, Section 5193 (e)(2)(B)9}

Liquid disinfectant traps and HEPA filtered vacuum lines prevent inadvertent contamination resulting from a release or backflow of liquid HIV/HBV contamination through a laboratory vacuum line.