1. Airflow shall be from low hazard to high hazard areas.

_{Good Practice}

_{CDC-NIH Biosafety in Microbiological and Biomedical Laboratories}

_{Prudent Practices in the Laboratory 8.C, 8.D}

_{NFPA 45,6.4.4}

Anterooms may be necessary for certain applications, such as clean rooms or tissue culture rooms. Potentially harmful aerosols can escape from the containment of the laboratory room unless the room air pressure is negative to adjacent non-laboratory areas.

It is recommended that laboratories should contain a fully integrated laboratory control system to control the temperature, ventilation rate and room pressurization. The control system should constantly monitor the amount of supply and exhaust air for the laboratory rooms and regulate the flow to maintain a net negative pressurization.

2. An adequate supply of make up air (90% of exhaust) should be provided to the lab.

_{Good Practice per Stanford University}

3. An air lock or vestibule may be necessary in certain high-hazard laboratories to minimize the volume of supply air required for negative pressurization control. These doors should be provided with interlocks so that both doors cannot open at the same time.

_{Good Practice per Stanford University}

4. A corridor should not be used as a plenum.

_{Good Practice per Stanford University}